Typical protein purification uses various chromatography and UF/DF techniques. Pictured is an AKTA Pilot which can handle flow rates in the 100-300mL/min range. The various lines would be attached to a column and inserted into containers of buffers and other solutions used to wash, bind, strip and or elute the desired substance.


A photometer used for the measurement of the absorbance of light in solutions, gases, or transparent or opaque solids, such as polished glass. Typically used at 280nm to determine the concentration of proteins related to antibody production.