A technique using various sized columns and substrates to perform purification by Size Exclusion Chromatography (SEC), Cation Exchange or Affinity Chromatography. The photo shows a Protein A 5ml column that is showing signs of channeling. The darker brown colored sample can be seen seeping unevenly through the resin. When you have a channeling column you … Read more
Ion chromatography is also referred to as affinity chromatography.
Typical protein purification uses various chromatography and UF/DF techniques. Pictured is an AKTA Pilot which can handle flow rates in the 100-300mL/min range. The various lines would be attached to a column and inserted into containers of buffers and other solutions used to wash, bind, strip and or elute the desired substance.